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Image Search Results
Journal:
Article Title: Improved Nla III digestion of PAGE-purified 102 bp ditags by addition of a single purification step in both the SAGE and microSAGE protocols
doi:
Figure Lengend Snippet: SYBR Green I stained polyacrylamide gels (12% PAGE in A and B, 8% PAGE in C) showing the efficacy of the purification step in the microSAGE procedure for untreated PC12 cells. Prior to the use of Clontech spe10 spin gel filtration columns, 26 bp ditags are not detectable (A). As shown above in Figure 1 for SAGE, DNA purification through the columns remarkably improves NlaIII digestion (B) and facilitates formation of concatemers (C). M1, 20 bp DNA ladder; M2, 100 bp DNA ladder; M3, 1 kb DNA ladder.
Article Snippet: Prior to the use of
Techniques: SYBR Green Assay, Staining, Purification, Filtration, Paired-end Tag, DNA Purification